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mlpa  (Beijing Genomics Institute Shenzhen)

 
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    Beijing Genomics Institute Shenzhen mlpa
    Mlpa, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mlpa/mlpa/med_rxiv__2025__10__24__25338696-37-5-13
    Average 86 stars, based on 1 article reviews
    mlpa - by Bioz Stars, 2026-09
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    Article Title: High-Resolution Variant Profiling of CAH-Associated Genes Using a Long-Read Sequencing Assay
    Article Snippet: 19 Samples were identified through MLPA and sanger at the clinical laboratory of Beijing Genomics Institution, China, and other 40 Samples were identified at Shengjing Hospital of China Medical University.



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    In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based <t>(MLPA</t> and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.
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    In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based (MLPA and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.

    Journal: Advanced Science

    Article Title: Large‐Scale Production of Transfusion‐Ready Red Blood Cells From Induced Pluripotent Stem Cells

    doi: 10.1002/advs.202504725

    Figure Lengend Snippet: In vitro assays confirming functional, definitive erythroid characteristics of the terminally differentiated iRBCs. A) Cytospins combined with benzidine‐giemsa staining on filter‐purified RETs and on nERYs. B) RET‐size measurement by flow cytometry on CD235 + /DRAQ5 ‐ cell population. C) Marker expression pattern of the iRBCs using flow cytometry, n ≥ 58. D) Quantification of Hbe (α2ε2), HbF (α2γ2) and HbA (α2β2) based on HPLC tracks performed on iRBCs, n = 8. E) Oxygen dissociation curve of iRBC lines measured by the Hemox analyzer. F) Deformability assay or RETs and nERY measured by ARCA. G) Blood group phenotype measured on iPSC lines by DNA‐based (MLPA and SNP array)‐ and on iRBCs by protein‐based (flow cytometry)‐ assays, n ≥ 3.

    Article Snippet: The multiplex ligation‐dependent probe amplification (MLPA) and SNP array were carried out by the Sanquin Diagnostic Department (Amsterdam, The Netherlands).

    Techniques: In Vitro, Functional Assay, Staining, Purification, Flow Cytometry, Marker, Expressing